Cloning by somatic cell nuclear transfer (SCNT) is an assisted reproductive technique with several applications in livestock. However, it is still a technique that shows low efficiency due to the high frequency of fetal and placental abnormalities. The somatic genome must be correctly reprogrammed after nuclear transfer to achieve normal embryo development. However, in cloned embryos this reprogramming does not occur efficiently, leading to changes in DNA methylation patterns and consequently alterations in gene expression profile of important genes for embryo and foetal development. In this study, we determined the mRNA levels for six target genes (DNMT1, DNMT3A, DNMT3B, TET1, TET2 and TET3) related to DNA methylation reprogramming in the ...
Background Successful reprogramming of a somatic genome to produce a healthy clone by somatic cells ...
Cloning through somatic cell nuclear transfer (SCNT) remains highly inefficient twenty years after t...
AbstractDNA methylation patterns are dynamic in cleavage-stage embryos of a number of mammalian spec...
Somatic cell nuclear transfer (SCNT) is a commercially available technique of assisted reproductive ...
This thesis is subdivided into three chapters and annexes: • Chapter 1: Theoretical rationale • Ch...
Somatic Cell Nuclear Transfer (SCNT- Cloning) is a technique that is based on the production of gene...
Somatic Cell Nuclear Transfer (SCNT), commonly known as cloning, is the transfer of a somatic nucleu...
Evidence indicates that failure of nuclear transfer (NT) embryos to develop normally can be attribut...
Embryos derived by somatic cell nuclear transfer (SCNT) produce few pregnancies that result in a liv...
AbstractWhile somatic cell nuclear transfer (SCNT) techniques have been successfully implemented in ...
<div><p>Incomplete epigenetic reprogramming is postulated to contribute to the low developmental suc...
Incomplete epigenetic reprogramming is postulated to contribute to the low developmental success fol...
To gain a better understanding of global methylation differ-ences associated with development of nuc...
During the process of cloning using nuclear transfer, epigenetic marks in cells must go through a re...
The aberrant expression of DNA methyltransferase 1 (DNMT1) in cloned embryos has been implicated as ...
Background Successful reprogramming of a somatic genome to produce a healthy clone by somatic cells ...
Cloning through somatic cell nuclear transfer (SCNT) remains highly inefficient twenty years after t...
AbstractDNA methylation patterns are dynamic in cleavage-stage embryos of a number of mammalian spec...
Somatic cell nuclear transfer (SCNT) is a commercially available technique of assisted reproductive ...
This thesis is subdivided into three chapters and annexes: • Chapter 1: Theoretical rationale • Ch...
Somatic Cell Nuclear Transfer (SCNT- Cloning) is a technique that is based on the production of gene...
Somatic Cell Nuclear Transfer (SCNT), commonly known as cloning, is the transfer of a somatic nucleu...
Evidence indicates that failure of nuclear transfer (NT) embryos to develop normally can be attribut...
Embryos derived by somatic cell nuclear transfer (SCNT) produce few pregnancies that result in a liv...
AbstractWhile somatic cell nuclear transfer (SCNT) techniques have been successfully implemented in ...
<div><p>Incomplete epigenetic reprogramming is postulated to contribute to the low developmental suc...
Incomplete epigenetic reprogramming is postulated to contribute to the low developmental success fol...
To gain a better understanding of global methylation differ-ences associated with development of nuc...
During the process of cloning using nuclear transfer, epigenetic marks in cells must go through a re...
The aberrant expression of DNA methyltransferase 1 (DNMT1) in cloned embryos has been implicated as ...
Background Successful reprogramming of a somatic genome to produce a healthy clone by somatic cells ...
Cloning through somatic cell nuclear transfer (SCNT) remains highly inefficient twenty years after t...
AbstractDNA methylation patterns are dynamic in cleavage-stage embryos of a number of mammalian spec...