Fractionation comparison of Persian Gulf jellyfish nematocyst venom by two methods of chromatography

In this paper, the nematocyst venom of the crambionella orsini jellyfish was fractionated by sizeexclusion and anion-exchange chromatography. Crambionella orsini is a jellyfish common to the Persian Gulf. The results of the mentioned methods have been investigated. The crambionella orsini’s venom has a hemolytic effect, which is similar to the other species. After the extraction of the nematocyst venom, the crude venom was partially purified using sephadex G-200 gel filtration and DEAE anion exchange chromatography. Protein elution was monitored by UV detection at 28 0nm. To determine the hemolytic fraction, every fraction was injected to 3 mice via their tail vein. Finally, all the data from both chromatography methods were compared. The gel filtrations second fraction and the first and second fractions of the anion exchange chromatography showed hemolytic activity. Determining an appropriate method for the purification of this venom can help find a comprehensive method for other marine venoms, especially jellyfish venoms, and may eventually help find specific antidotes for the stings of jellyfish of these species