Add to ORKGUnderstanding how microenvironmental cues influence cellular behavior will enable development of efficient and robust pluripotent stem cell differentiation protocols. Unlike traditional cell culture dishes, microfluidic bioreactors can provide stable microenvironmental conditions by continuous medium perfusion at a controlled rate. The aim of this study is to investigate whether a microfluidic culture device could be used as a perfused platform for long‐term cell culture processes such as the retinal differentiation of human induced pluripotent stem cells. The perfusion flow rate is established based on the degradation and consumption of growth factors (DKK‐1, Noggin, IGF‐1, and bFGF) and utilizing the Péclet number. The device's performanc...
Cell cycle and differentiation precisely regulate the size and geometry of tissues and organs, and a...
Biomolecular signaling is of utmost importance in governing many biological processes such as the pa...
BACKGROUND: The use of human pluripotent stem cell-derived retinal cells for cell therapy strategies...
Thesis (Ph. D.)--Harvard-MIT Division of Health Sciences and Technology, 2008.Includes bibliographic...
The development of in vitro models to screen the effect of different concentrations, combinations an...
Human induced pluripotent stem cells (hiPSC) have the potential to provide patient and disease speci...
During development and regeneration, tissues emerge from coordinated sequences of stem cell renewal,...
In early embryonic development, spatial gradients of diffusible signaling molecules play important r...
High throughput experiments can be used to spatially and temporally investigate the many factors tha...
Conventional culture systems are often limited in their ability to regulate the growth and different...
The capacity of milli and micro litre bioreactors to accelerate process development has been success...
Background: Microfluidics is an enabling technology with a number of advantages over traditional tis...
In this thesis, effects of stable concentration of key growth factors on retinal differentiation of ...
The ability of pluripotent stem cells to differentiate into any of the three germ layers have sparke...
The capacity of pluripotent stem cells both for self-renewal and to differentiate into any cell type...
Cell cycle and differentiation precisely regulate the size and geometry of tissues and organs, and a...
Biomolecular signaling is of utmost importance in governing many biological processes such as the pa...
BACKGROUND: The use of human pluripotent stem cell-derived retinal cells for cell therapy strategies...
Thesis (Ph. D.)--Harvard-MIT Division of Health Sciences and Technology, 2008.Includes bibliographic...
The development of in vitro models to screen the effect of different concentrations, combinations an...
Human induced pluripotent stem cells (hiPSC) have the potential to provide patient and disease speci...
During development and regeneration, tissues emerge from coordinated sequences of stem cell renewal,...
In early embryonic development, spatial gradients of diffusible signaling molecules play important r...
High throughput experiments can be used to spatially and temporally investigate the many factors tha...
Conventional culture systems are often limited in their ability to regulate the growth and different...
The capacity of milli and micro litre bioreactors to accelerate process development has been success...
Background: Microfluidics is an enabling technology with a number of advantages over traditional tis...
In this thesis, effects of stable concentration of key growth factors on retinal differentiation of ...
The ability of pluripotent stem cells to differentiate into any of the three germ layers have sparke...
The capacity of pluripotent stem cells both for self-renewal and to differentiate into any cell type...
Cell cycle and differentiation precisely regulate the size and geometry of tissues and organs, and a...
Biomolecular signaling is of utmost importance in governing many biological processes such as the pa...
BACKGROUND: The use of human pluripotent stem cell-derived retinal cells for cell therapy strategies...