Detection of Salmonella enterica, Escherichia coli O157 and Listeria monocytogenes through bead based Magpix® fluidics

The research is testing the limits of detection and multiplexing with the use of three pathogens with the machine created from Luminex called Magpix. The pathogens are Salmonella enterica, Listeria monocytogenes, and Escherichia Coli O157. The gene that is being focused on for each pathogen is invA for Salmonella, hlyA for listeria, and hlyA for Escherichia Coli. In this research, we started with prepared probes that were attached to the magnetic microsphere beads. Then we extracted and amplify the DNA. Furthermore, the probes were combined to the amplified DNA and used this in Magpix which gave a corresponding MFI value