Real-time analysis of cell-surface adhesive interactions using TSM sensor

Poster presented at Biomedical Technology Showcase 2006, Philadelphia, PA. Retrieved 18 Aug 2006 from http://www.biomed.drexel.edu/new04/Content/Biomed_Tech_Showcase/Poster_Presentations/Lec_7.pdf.Cell adhesion process and the molecular interactions that determine its kinetics were investigated using Thickness Shear Mode (TSM) sensor. The goal of this study was to correlate sensor readings with the progression of cell adhesion. In particular, the specific effects of receptor-mediated adhesion, the glycocalyx, and surface charge on initial cell-surface attachment and steady state adhesion of endothelial cells were investigated. We found a strong correlation between magnitude changes (DMag) and the development of cell adhesion strength by comparing the sensor readings with independently assessed cell adhesion. The result showed that integrin binding determines the kinetics of initial cell attachment while heparan sulfate proteoglycan (HSPG) modulates steady state adhesion strength. Coating the sensor surface with the positively charged poly-D-lysine (PDL) enhanced the initial interaction with substratum