Data from: How quantitative is metabarcoding: a meta-analytical approach
- Lamb, Philip D
- Hunter, Ewan
- Pinnegar, John K
- Creer, Simon
- Davies, Richard G
- Taylor, Martin I.
DOIAbstract
Metabarcoding has been used in a range of ecological applications such as taxonomic assignment, dietary analysis, and the analysis of environmental DNA. However, after a decade of use in these applications there is little consensus on the extent to which proportions of reads generated corresponds to the original proportions of species in a community. To quantify our current understanding we conducted a structured review and meta-analysis. The analysis suggests that a weak quantitative relationship may exist between the biomass and sequences produced (slope =0.52 ±0.34, p<0.01)), albeit it with a large degree of uncertainty. None of the tested moderators: sequencing platform type, the number of species used in a trial, or the source of DNA w...
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