Annual forage samples sent by producers to Ward Laboratories Inc. for nitrate analysis were evaluated to determine which cover crop species in Nebraska are most likely to accumulate nitrates, and how often the accumulated nitrates are considered toxic by traditional recommendations. Additionally, nitrate levels of cover crop mixes grown in research trials were analyzed to ensure species differences were repeated when grown together in the same fields. Brassicas accumulate more nitrate than small grains, millet, sorghum/sudan grasses, or cover crop mixes. Brassicas accumulated levels of nitrate considered moderately to highly toxic in 48% of the samples. The other cover crop species accumulated potentially toxic nitrate levels in 20–28% of t...