The Molecular Assembly of Amyloid A beta Controls Its Neurotoxicity and Binding to Cellular Proteins

Accumulation of beta-sheet-rich peptide (A beta) is strongly associated with Alzheimer's disease, characterized by reduction in synapse density, structural alterations of dendritic spines, modification of synaptic protein expression, loss of long-term potentiation and neuronal cell death. A beta species are potent neurotoxins, however the molecular mechanism responsible for A beta toxicity is still unknown. Numerous mechanisms of toxicity were proposed, although there is no agreement about their relative importance in disease pathogenesis. Here, the toxicity of A beta 1-40 and A beta 1-42 monomers, oligomers or fibrils, was evaluated using the N2a cell line. A structure-function relationship between peptide aggregation state and toxic properties was established. Moreover, we demonstrated that A beta toxic species cross the plasma membrane, accumulate in cells and bind to a variety of internal proteins, especially on the cytoskeleton and in the endoplasmatic reticulum (ER). Based on these data we suggest that numerous proteins act as A beta receptors in N2a cells, triggering a multi factorial toxicity