An examination (analysis) of susceptibilities to antibiotics of the strain Staphylococcus aureus performed by phenotypic methods in conventional microbiological laboratory and interpretation of its results.

The aim of bachelor´s thesis is the understanding and the correct interpretation of results of susceptibility to antibiotics of the strain Staphylococcus aureus from serious clinical materials as a hemoculture, a peritoneal fluid from joints and abscesses, a smear from wounds, tissues during infectious total artificial joints, samples of an urine, materials from the lower respiratory tract and another clinical samples. According to data which have been used, my aim also have been to prove or eventually disprove the first hypothesis, that makes sense to collect also hemocultures from patient whose result in the serious clinical material contained the strain of Staphylococcus aureus. Prove or disprove the second hypothesis that with the number of positive results of the strain of Staphylococcus aureus in samples of urine will be in the same time the most positive hemocultures in percentage in comparison with results of the strains of Staphylococcus aureus in other clinical materials. Defined targets were reached by regular practical education in the Department of Clinical Microbiology. I had adopted a methodology of examination of susceptibility in the department and then have practiced under the professional supervision. Before the practical part of my thesis, I have had to elaborate and learn theoretical part of it. In that part is deeply described the kind Staphylococcus aureus, antibiotical treatment of this strain and the list of mostly tested antibiotics in the routine microbiological laboratory. In the practical part of the thesis I am focusing on the determination of sensitivity to antibiotics by the disk diffusion method and a defining minimal inhibition concentration. I have interpreted results and for example, I have depicted mostly occurred antibiograms and equally less occurred or rare ones. Then I have processed results of sensitivity of Staphylococcus aureus. The examined set has been containing 588 samples of positive Staphylococcus aureus. 72 samples from the set have been MRSA positive. A representation of positive hemocultures has been examined too in currently or earlier found positive materials. Positive hemocultures in positive materials have been found in ca 5,2 % of all positive clinical materials. The most positive hemocultures were found in previous positive samples of urine ca 17 %. The second highest positivity of hemocultures was in samples from bones and joints ca 14 %. Then artificial materials ca 13 %, venous catheters ca 10 %, abscess ca 6 %, materials from respiratory tract ca 4 %, soft tissues ca 3 % and wounds ca 2 %. The significant gained finding and conclusion of the thesis is the fact that during serious staphylococcus infections the sampling of hemocultures is indicating as a necessity and as an obligation of the sampling of hemocultures of a patient is finding of the strain of Staphylococcus aureus in the sample of urine