Preparation of drugs in nanoparticles for targeted treatment of cancer

The aim of this thesis was to study the ability of the apoferritin to encapsulate drugs vandetanib and etoposid. Vandetanib is an anticancer drug used for the treatment of tumors of the thyroid gland. It acts as an inhibitor of tyrosine kinase receptor. Etoposid, which is widely used for the treatment of malignant blood diseases or solid tumors inhibits type II topoisomerase that regulates topology of DNA. Targeting treatment with nanoparticles can minimize adverse effects connected with both drugs. An apoferritin is a naturally occurring protein that is composed of 24 ferritin subunits. Its structure creates an internal cavity that can be loaded by any compoundands. The structure is remarkably stable and is able to withstand biologically extreme temperatures (up to 70řC) and a wide pH range (pH 2-10). Furthermore, apoferritin can move undetected through the body without any immune response. It is also possible to modify its surface by ligands specific for targeting tissues. The effect of the concentration of apoferritin and the effect of the concentration of the drug on the final concentration of the encapsulated drug was studied. In addition, even the effectiveness of encapsulation was studied. In the case of vandetanib, at a constant concentration of drug and an increasing concentration of..