This bachelor's thesis deals with the design and implementation of the software part of the system for protection against DoS attacks. Nowadays Denial of Service attacks are quite common and can cause significant financial damage to internet or service providers. The main goal of this thesis was to provide software, which is focused on high-speed data throughput and can provide efficient protection against these attacks in 100 Gbps networks. Key part of the system, which is being developed in cooperation with CESNET, is hardware-accelerated network interface card, which can process incoming network traffic at full wire-speed and does the operations laid down by the software part. The main task of the software is evaluation of the informati...