3’ uridylation is increasingly recognized as a conserved RNA modification process associated with RNA turnover in eukaryotes. 2’-O-methylation on the 3’ terminal ribose protects micro(mi)RNAs from 3’ truncation and 3’ uridylation in Arabidopsis. Previously, we identified HESO1 as the nucleotidyl transferase that uridylates most unmethylated miRNAs in vivo, but substantial 3’ tailing of miRNAs still remains in heso1 loss-of-function mutants. In this study, we found that among nine other potential nucleotidyl transferases, UTP:RNA URIDYLYLTRANSFERASE 1 (URT1) is the single most predominant nucleotidyl transferase that tails miRNAs. URT1 and HESO1 prefer substrates with different 3’ end nucleotides in vitro and act cooperatively to tail differ...
Regulation of gene expression by small RNAs (~20–30 nucleotides in length) plays an essential role i...
Terminal nucleotidyl transferases (TENTs) generate miRNA isoforms (isomiRs) by posttranscriptional 3...
<div><p>Post-transcriptional modifications of miRNAs with 3′ non-templated nucleotide additions (NTA...
3’ uridylation is increasingly recognized as a conserved RNA modification process associated with RN...
Publisher's PDF.3’ uridylation is increasingly recognized as a conserved RNA modification process as...
All types of small RNAs in plants, piwi-interacting RNAs (piRNAs) in animals and a subset of siRNAs ...
All types of small RNAs in plants, piwi-interacting RNAs (piRNAs) in animals and a subset of siRNAs ...
In plants, methylation catalyzed by HEN1 (small RNA methyl transferase) prevents microRNAs (miRNAs) ...
HEN1-mediated 2′-O-methylation has been shown to be a key mechanism to protect plant microRNAs (miRN...
SummaryHEN1-mediated 2′-O-methylation has been shown to be a key mechanism to protect plant microRNA...
In plants, methylation catalyzed by HEN1 (small RNA methyl transferase) prevents microRNAs (miRNAs) ...
<p>Nucleotidyl transferase assays were conducted with recombinant URT1, HESO1, or no enzyme as indic...
After transcription, a large number of cellular RNAs employ modifications to increase their diversit...
SummarySmall RNAs of 21–25 nucleotides (nt), including small interfering RNAs (siRNAs) and microRNAs...
Plant small RNAs are 3\u27 methylated by the methyltransferase HUA1 ENHANCER1 (HEN1). In plant hen1 ...
Regulation of gene expression by small RNAs (~20–30 nucleotides in length) plays an essential role i...
Terminal nucleotidyl transferases (TENTs) generate miRNA isoforms (isomiRs) by posttranscriptional 3...
<div><p>Post-transcriptional modifications of miRNAs with 3′ non-templated nucleotide additions (NTA...
3’ uridylation is increasingly recognized as a conserved RNA modification process associated with RN...
Publisher's PDF.3’ uridylation is increasingly recognized as a conserved RNA modification process as...
All types of small RNAs in plants, piwi-interacting RNAs (piRNAs) in animals and a subset of siRNAs ...
All types of small RNAs in plants, piwi-interacting RNAs (piRNAs) in animals and a subset of siRNAs ...
In plants, methylation catalyzed by HEN1 (small RNA methyl transferase) prevents microRNAs (miRNAs) ...
HEN1-mediated 2′-O-methylation has been shown to be a key mechanism to protect plant microRNAs (miRN...
SummaryHEN1-mediated 2′-O-methylation has been shown to be a key mechanism to protect plant microRNA...
In plants, methylation catalyzed by HEN1 (small RNA methyl transferase) prevents microRNAs (miRNAs) ...
<p>Nucleotidyl transferase assays were conducted with recombinant URT1, HESO1, or no enzyme as indic...
After transcription, a large number of cellular RNAs employ modifications to increase their diversit...
SummarySmall RNAs of 21–25 nucleotides (nt), including small interfering RNAs (siRNAs) and microRNAs...
Plant small RNAs are 3\u27 methylated by the methyltransferase HUA1 ENHANCER1 (HEN1). In plant hen1 ...
Regulation of gene expression by small RNAs (~20–30 nucleotides in length) plays an essential role i...
Terminal nucleotidyl transferases (TENTs) generate miRNA isoforms (isomiRs) by posttranscriptional 3...
<div><p>Post-transcriptional modifications of miRNAs with 3′ non-templated nucleotide additions (NTA...