Crystallization of calcium phosphates on carbonatic substrates after ammonium phosphate consolidating treatments

Diammonium hydrogenphosphate (DAP, (NH4)2HPO4) is a promising candidate for consolidating treatment for carbonatic substrates of decayed ornamental stones. The treatment gives rise to the formation of calcium phosphates through a mild reaction of dissolution and re-crystallization1, which takes place between hydrogen phosphate groups of the reagent and calcium ions of calcite of the substrate. Calcium phosphates nucleate on calcite boundaries with a pseudomorphic replacement reaction2, and grow on pre-reacted profiles of calcite grains. In recent investigations3, it was observed that the reaction of DAP solutions with calcite induces the formation of calcium phosphates in a multiphase assemblage. The formation of specific crystalline phases and their arrangements on the stone substrate governs the performance of inorganic-mineral consolidating treatments. It follows that the survey of the mechanism that rules the formation of calcium phosphates on calcite is crucial in conservation field but their investigation, especially when in mixtures, is an analytical challenge. In this work, we present a new multi-analytical approach in order to characterize the newly-formed crystalline phases and their distribution on calcite of the substrate. Our experimental results show that the DAP reaction with calcite forms stable hydroxyapatite (HAP, (Ca5(PO4)3OH) and metastable brushite (DCPD, CaHPO4\ub72H2O) and octacalcium phosphate (OCP, Ca8(HPO4)2\ub75H2O) as well as amorphous compounds (e.g., ACP, amorphous calcium phosphate, CaxHy(PO4)z\ub7nH2O, with n = 3\u20134.5). The formation of specific phases and their morphology seems to be directly influenced by the duration of the treatment, by the microstructure of the stone matrix and by the DAP molarity. Moreover, the pH variation during the reaction affects the growth of HAP and OCP, inducing the formation of inter-layered sub-micrometric individuals. This research supplies a new analytical approach for the characterization of calcium phosphates multi-phase mixture and it paves the way to a critical evaluation of DAP treatments applied on Cultural Heritage surfaces