The binding of transcription factors (TFs) to their regulatory sites on DNA determines how much and how timely a particular gene will be expressed, and ultimately how the cell respond to external cues. TF binding is typically studied by bulk biochemical experiments as chromatin immunoprecipitation (ChIP) As these methods provide limited temporal resolution and they are unable to provide information about these interactions at the single cell level, the interpretation of ChIP results can be challenging when dealing with TFs exhibiting rapid turnover, or with cells and tissues exhibiting a patterned nonhomogeneous transcriptional response to an external stimulus. Here we describe a microscopy-based single molecule imaging approach which can b...
<p>DNA damage in cells occurs from both endogenous and exogenous sources, and failure to repair such...
Different proteins and complexes work together at multiple time scales to orchestrate the activation...
The tumor suppressor p53, one of the most intensely investigated proteins, is usually studied by exp...
Live-cell microscopy has highlighted that transcription factors bind transiently to chromatin but it...
p53 can adopt two forms in vitro, a latent form that binds naked DNA poorly and an active form that ...
p53 can adopt two forms in vitro, a latent form that binds naked DNA poorly and an active form that ...
Transcription factor (TF) binding to regulatory sequences is the essential step of gene regulation, ...
The transcription factors NF-κB and p53 are key regulators in the genotoxic stress response and are ...
Transcription factors (TFs) regulate gene expression in both prokaryotes and eukaryotes by recognizi...
Understanding the principles governing mammalian gene regulation has been hampered by the difficulty...
A cell has to react to a multitude of different extrinsic and intrinsic stress signals on a daily ba...
Transcription factors (TFs) regulate gene expression in both prokaryotes and eukaryotes by recognizi...
The tumor suppressor p53 is activated by stress and leads to cellular outcomes such as apoptosis and...
Proper timing of gene expression requires that transcription factors (TFs) efficiently locate and bi...
SummaryThe tumor suppressor p53 is activated by stress and leads to cellular outcomes such as apopto...
<p>DNA damage in cells occurs from both endogenous and exogenous sources, and failure to repair such...
Different proteins and complexes work together at multiple time scales to orchestrate the activation...
The tumor suppressor p53, one of the most intensely investigated proteins, is usually studied by exp...
Live-cell microscopy has highlighted that transcription factors bind transiently to chromatin but it...
p53 can adopt two forms in vitro, a latent form that binds naked DNA poorly and an active form that ...
p53 can adopt two forms in vitro, a latent form that binds naked DNA poorly and an active form that ...
Transcription factor (TF) binding to regulatory sequences is the essential step of gene regulation, ...
The transcription factors NF-κB and p53 are key regulators in the genotoxic stress response and are ...
Transcription factors (TFs) regulate gene expression in both prokaryotes and eukaryotes by recognizi...
Understanding the principles governing mammalian gene regulation has been hampered by the difficulty...
A cell has to react to a multitude of different extrinsic and intrinsic stress signals on a daily ba...
Transcription factors (TFs) regulate gene expression in both prokaryotes and eukaryotes by recognizi...
The tumor suppressor p53 is activated by stress and leads to cellular outcomes such as apoptosis and...
Proper timing of gene expression requires that transcription factors (TFs) efficiently locate and bi...
SummaryThe tumor suppressor p53 is activated by stress and leads to cellular outcomes such as apopto...
<p>DNA damage in cells occurs from both endogenous and exogenous sources, and failure to repair such...
Different proteins and complexes work together at multiple time scales to orchestrate the activation...
The tumor suppressor p53, one of the most intensely investigated proteins, is usually studied by exp...