We present a baculovirus-based protein engineering method that enables site-specific introduction of unique functionalities in a eukaryotic protein complex recombinantly produced in insect cells. We demonstrate the versatility of this efficient and robust protein production platform, \u2018MultiBacTATAG\u2019, (i) for the fluorescent labeling of target proteins and biologics using click chemistries, (ii) for glycoengineering of antibodies, and (iii) for structure\u2013function studies of novel eukaryotic complexes using single-molecule F\uf6rster resonance energy transfer as well as site-specific crosslinking strategies
Abstract Background Baculovirus-mediated expression in insect cells is a powerful approach for prote...
The genetic code can be expanded to include unnatural amino acids (Uaas) by engineering orthogonal c...
"Recombinant protein production plays a pivotal role in the industrial manufacturing of t...
International audienceMost essential activities in eukaryotic cells are catalyzed by large multiprot...
The production of proteins in sufficient quantity and of appropriate quality is an essential pre-req...
The baculovirus/insect cell system (BICS) is widely used in academia and industry to produce eukaryo...
Biocomplexity—the study of complex structures and behaviours that take place in biological systems—g...
Abstract The MultiBac baculovirus/insect cell expression vector system was conceived as a user-frien...
Proteins are the workhorses of the cell, playing crucial roles in virtually every biological process...
SummaryThe concept of the cell as a collection of multisubunit protein machines is emerging as a cor...
More than one third of all proteins are metalloproteins. They catalyze important reactions such as p...
The rational engineering of proteins is driven by contemporary needs for new and altered biomolecula...
Genetic code expansion is emerging as an important tool for manipulation and labeling of proteins in...
Proteins, large biological molecules synthesized by living organisms, serve a wide array of function...
Although chemists can synthesize virtually any small organic molecule, our ability to rationally man...
Abstract Background Baculovirus-mediated expression in insect cells is a powerful approach for prote...
The genetic code can be expanded to include unnatural amino acids (Uaas) by engineering orthogonal c...
"Recombinant protein production plays a pivotal role in the industrial manufacturing of t...
International audienceMost essential activities in eukaryotic cells are catalyzed by large multiprot...
The production of proteins in sufficient quantity and of appropriate quality is an essential pre-req...
The baculovirus/insect cell system (BICS) is widely used in academia and industry to produce eukaryo...
Biocomplexity—the study of complex structures and behaviours that take place in biological systems—g...
Abstract The MultiBac baculovirus/insect cell expression vector system was conceived as a user-frien...
Proteins are the workhorses of the cell, playing crucial roles in virtually every biological process...
SummaryThe concept of the cell as a collection of multisubunit protein machines is emerging as a cor...
More than one third of all proteins are metalloproteins. They catalyze important reactions such as p...
The rational engineering of proteins is driven by contemporary needs for new and altered biomolecula...
Genetic code expansion is emerging as an important tool for manipulation and labeling of proteins in...
Proteins, large biological molecules synthesized by living organisms, serve a wide array of function...
Although chemists can synthesize virtually any small organic molecule, our ability to rationally man...
Abstract Background Baculovirus-mediated expression in insect cells is a powerful approach for prote...
The genetic code can be expanded to include unnatural amino acids (Uaas) by engineering orthogonal c...
"Recombinant protein production plays a pivotal role in the industrial manufacturing of t...