Soft X-ray contact microscopy of nematode Caenorhabditis elegans
- G. Poletti
- F. Orsini
- D. Batani
- A. Bernardinello
- T. Desai
- J. Ullschmied
- J. Skala
- B. Kralikova
- E. Krousky
- L. Juha
- M. Pfeifer
- C. Kadlec
- T. Mocek
- A. Prä
- O. Renner
- F. Cotelli
- C. Lora Lamia
- A. Zullini
Publication date
January 2004
DOI Publisher
Springer Science and Business Media LLCAbstract
Soft X-ray Contact Microscopy (SXCM) of Caenorhabditis elegans nematodes with typical length 3c800\u3bc m and diameter 3c30\u3bc m has been performed using the PALS laser source of wavelength \u3bb=1.314\u3bc m and pulse duration \u3c4(FWHM)=400 ps. Pulsed soft X-rays were generated using molybdenum and gold targets with laser intensities I 651014 W/cm2. Images have been recorded on PMMA photo resists and analyzed using an atomic force microscope operating in contact mode. Cuticle features and several internal organs have been identified in the SXCM images including lateral field, cuticle annuli, pharynx, and hypodermal and neuronal cell nuclei
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