Nicotinamide adenine dinucleotide phosphate-dependent 7\u3b1- hydroxysteroid dehydrogenase (7\u3b1-HSDH) and 7\u3b2-hydroxysteroid dehydrogenases (7\u3b2-HSDH) from Clostridium absonum catalyze the epimerization of primary bile acids through 7-keto bile acid intermediates and may be suitable as biocatalysts for the synthesis of bile acids derivatives of pharmacological interest. C. absonum 7\u3b1-HSDH has been purified to homogeneity and the N-terminal sequence has been determined by Edman sequencing. After PCR amplifications of a gene fragment with degenerate primers, cloning of the complete gene (786 nt) has been achieved by sequencing of C. absonum genomic DNA. The sequence coding for the 7\u3b2-HSDH (783 nt) has been obtained by sequenc...
Ursodeoxycholic acid, a secondary bile acid, is used as a drug for the treatment of various liver di...
Conversion of the primary bile acids cholic acid (CA) and chenodeoxycholic acid (CDCA) to the second...
Two enzymes, a 7α-hydroxysteroid dehydrogenase (7α-HSDH) and an NADH-dependent flavin oxidoreductase...
Bakonyi D, Hummel W. Cloning, expression, and biochemical characterization of a novel NADP(+)-depend...
Hydroxysteroid dehydrogenases (HSDHs), mainly obtained from bacterial sources, have been widely empl...
Southern blot analysis indicated that the gene encoding the constitutive, NADP-linked bile acid 7 al...
Hydroxysteroid dehydrogenases (HSDHs) are valuable biocatalysts for the regio- and stereoselective m...
The 7a-hydroxysteroid dehydrogenase (EC 1.1.1.159) gene from Eschenichia coli HB101 was cloned and e...
Clostridium absonum ATCC 27555 synthétise trois hydroxystéroïde déshydrogénases inductibles réalisan...
Clostridium absonum ATCC 27555 synthétise trois hydroxystéroïde déshydrogénases inductibles réalisan...
The paper reports the partial purification and characterization of the 7_- and 7_-hydroxysteroid deh...
A novel 7α-hydroxysteroid dehydrogenase (7α-HSD) was purified from Pseudomonas sp. B-0831. The molec...
Bile acids are detergent molecules that solubilize dietary lipids and lipid-soluble vitamins. Humans...
Bile acids are one group of steroids that can be metabolically transformed by human gut anaerobes. O...
We report the very efficient biotransformation of cholic acid to 7-keto- and 7,12-diketocholic acid...
Ursodeoxycholic acid, a secondary bile acid, is used as a drug for the treatment of various liver di...
Conversion of the primary bile acids cholic acid (CA) and chenodeoxycholic acid (CDCA) to the second...
Two enzymes, a 7α-hydroxysteroid dehydrogenase (7α-HSDH) and an NADH-dependent flavin oxidoreductase...
Bakonyi D, Hummel W. Cloning, expression, and biochemical characterization of a novel NADP(+)-depend...
Hydroxysteroid dehydrogenases (HSDHs), mainly obtained from bacterial sources, have been widely empl...
Southern blot analysis indicated that the gene encoding the constitutive, NADP-linked bile acid 7 al...
Hydroxysteroid dehydrogenases (HSDHs) are valuable biocatalysts for the regio- and stereoselective m...
The 7a-hydroxysteroid dehydrogenase (EC 1.1.1.159) gene from Eschenichia coli HB101 was cloned and e...
Clostridium absonum ATCC 27555 synthétise trois hydroxystéroïde déshydrogénases inductibles réalisan...
Clostridium absonum ATCC 27555 synthétise trois hydroxystéroïde déshydrogénases inductibles réalisan...
The paper reports the partial purification and characterization of the 7_- and 7_-hydroxysteroid deh...
A novel 7α-hydroxysteroid dehydrogenase (7α-HSD) was purified from Pseudomonas sp. B-0831. The molec...
Bile acids are detergent molecules that solubilize dietary lipids and lipid-soluble vitamins. Humans...
Bile acids are one group of steroids that can be metabolically transformed by human gut anaerobes. O...
We report the very efficient biotransformation of cholic acid to 7-keto- and 7,12-diketocholic acid...
Ursodeoxycholic acid, a secondary bile acid, is used as a drug for the treatment of various liver di...
Conversion of the primary bile acids cholic acid (CA) and chenodeoxycholic acid (CDCA) to the second...
Two enzymes, a 7α-hydroxysteroid dehydrogenase (7α-HSDH) and an NADH-dependent flavin oxidoreductase...