Synthesis and pharmacology of 3-hydroxy-\u3942-isoxazoline-cyclopentane analogues of glutamic acid

The synthesis and pharmacology of two potential glutamic acid receptor ligands are described. Preparation of the bicyclic 3-hydroxy-\u3942-isoxazoline-cyclopentane derivatives (\ub1)-7 and (\ub1)-8 was accomplished via 1,3-dipolar cycloaddition of bromonitrile oxide to suitably protected 1-amino-cyclopent-3-enecarboxylic acids. Their structure was established using a combination of 1H NMR spectroscopy and molecular mechanics calculations carried out on the intermediate cycloadducts (\ub1)-11 and (\ub1)-12. Amino acid derivatives (\ub1)-7 and (\ub1)-8 were assayed at ionotropic and metabotropic glutamic acid receptor subtypes and their activity compared with that of trans-ACPD and cis-ACPD. The results show that the replacement of the \u3c9-carboxylic group of the model compounds with the 3-hydroxy-\u3942-isoxazoline moiety abolishes or reduces drastically the activity at the metabotropic glutamate receptors. Conversely, on passing from cis-ACPD to derivative (\ub1)-8, the agonist activity at NMDA receptors is almost unaffected