Mobilized blood CD34+ cells from cancer patients were ex vivo infected by a recombinant adenovirus vector carrying an alkaline phosphatase gene, whose expression is evaluable by flow cytometry. A mean of 40% CD34+ cells were infected by the vector, with high levels of expression of the transgene. Among attempts to improve infection efficiency by manipulating culture conditions, only reinfection by the same vector achieved a 10% increase of transgene expression. Transduced CD34+ cells were induced to differentiate along the myeloid and the dendritic lineage, and in either case AP+ cells were detectable among the differentiated cell population. We conclude that adenovirus vectors may be useful tools for gene transduction into mobilized blood ...
Recombinant adenovirus serotype 5 (Ad5) vectors represent one of the most efficient gene delivery ve...
Replication-defective adenoviruses are effective vehicles for gene transfer, both for the repair of ...
A new procedure is described for the generation of high-titer, helper-free retrovirus vectors employ...
OBJECTIVE: To optimize transduction efficiency of mobilized CD34(+) cells with serotype-5 adenovirus...
With a view to using multiple injections of anticancer dendritic cell (DC)-based vaccines, we evalua...
In a search for means to deliver exogenous gene(s) into human dendritic cells (DCs) from the perspec...
Human bone marrow mononuclear cells (BMMNCs) and en-riched CD34 positive (CD34') cells were tra...
Immortalized cell lines have been used to study infection and replication of adeno-associated virus ...
Dendritic cells (DCs) are professional antigen presenting cells that are being considered as potenti...
Hematopoietic stem cells (HSCs) are defined by their capacity to self-renew and differentiate into a...
In the last decade adenovirus (AdV) vectors have emerged as promising technology in gene therapy. Th...
Recombinant adenovirus serotype 5 (Ad5) vectors represent one of the most efficient gene delivery ve...
Recombinant adenovirus serotype 5 (Ad5) vectors represent one of the most efficient gene delivery ve...
BACKGROUND:Hematopoietic stem cells (HSC), in particular mobilized peripheral blood stem cells, repr...
Recombinant adenovirus serotype 5 (Ad5) vectors represent one of the most efficient gene delivery ve...
Recombinant adenovirus serotype 5 (Ad5) vectors represent one of the most efficient gene delivery ve...
Replication-defective adenoviruses are effective vehicles for gene transfer, both for the repair of ...
A new procedure is described for the generation of high-titer, helper-free retrovirus vectors employ...
OBJECTIVE: To optimize transduction efficiency of mobilized CD34(+) cells with serotype-5 adenovirus...
With a view to using multiple injections of anticancer dendritic cell (DC)-based vaccines, we evalua...
In a search for means to deliver exogenous gene(s) into human dendritic cells (DCs) from the perspec...
Human bone marrow mononuclear cells (BMMNCs) and en-riched CD34 positive (CD34') cells were tra...
Immortalized cell lines have been used to study infection and replication of adeno-associated virus ...
Dendritic cells (DCs) are professional antigen presenting cells that are being considered as potenti...
Hematopoietic stem cells (HSCs) are defined by their capacity to self-renew and differentiate into a...
In the last decade adenovirus (AdV) vectors have emerged as promising technology in gene therapy. Th...
Recombinant adenovirus serotype 5 (Ad5) vectors represent one of the most efficient gene delivery ve...
Recombinant adenovirus serotype 5 (Ad5) vectors represent one of the most efficient gene delivery ve...
BACKGROUND:Hematopoietic stem cells (HSC), in particular mobilized peripheral blood stem cells, repr...
Recombinant adenovirus serotype 5 (Ad5) vectors represent one of the most efficient gene delivery ve...
Recombinant adenovirus serotype 5 (Ad5) vectors represent one of the most efficient gene delivery ve...
Replication-defective adenoviruses are effective vehicles for gene transfer, both for the repair of ...
A new procedure is described for the generation of high-titer, helper-free retrovirus vectors employ...