The action of adenosine deaminase (EC 3.5.4.4.) on adenosine and deoxyadenosine acetates : The crucial role of the 5 '-hydroxy group for the enzyme activity

From adenosine 1,2'-deoxyadenosine 3 and 3'-deoxyadenosine 5 all the acetates were prepared by lipase-catalyzed reactions. Only the acetates with free 5'-hydroxy group were deaminated by adenosine deaminase (ADA), confirming the crucial role of 5'-OH for the enzyme activity