Determination of myoseverin embryotoxic potential by using FETAX

BACKGROUND: Since MYS is a microtubular poison with a reversible activity, Xenopus blastulae were exposed to MYS to verify the eventual drug-related developmental suspension and the reversibility of this effect. METHODS: Lethal and teratogenic effects of myoseverin (MYS) were evaluated using the FETAX Embryos were exposed to different MYS concentrations from stage 8 to stage 47. RESULTS: Probit analysis gave 12.14 mu M LC50 and 7.67 mu M TC50 from which 1.58 T.I. is derived. Several malformations were observed such as facial abnormalities, abnormal tail flexure, heart ventricle chamber enlargement and external appendix. MYS led to an arrest of living embryo development. Before MYS removing, exposed blastulae showed the lack of mitotic spindles along with different nuclei alterations. Living embryos, moved in control solution, mainly died around the hatching showing severe malformations likely ascribable to the altered planes of newly occurring mitosis. CONCLUSION: In spite of the low T.I, MYS has to be considered a highly teratogenic compound