Add to ORKGalpha-Lactalbumin (alpha-LA) is a regulatory protein by which the mammalian beta1,4-galactosyltransferase (beta1,4-galT) is induced to utilize glucose as an acceptor instead of N-acetylglucosamine (GlcNAc) during lactose synthesis in mammary gland. alpha-LA can also modulate beta1,4-galT to utilize UDP-N-acetylgalactosamine (UDP-GalNAc) as a donor towards GlcNAc acceptor substrate with high efficiency in vitro [Do, Do and Cummings (1995) J. Biol. Chem. 270, 18447-18451]. In the present study we transfected cDNA encoding bovine alpha-LA into Lec8 cells and examined whether nucleotide sugar switching of UDP-galactose (UDP-Gal) into UDP-GalNAc occurred in vivo and whether the neo-glycosylation of GalNAcbeta1,4GlcNAc-R structure was synthesized...
<p>BMEC were treated for 12 h with various concentration of glucose. Values with different superscri...
As the main precursor for lactose synthesis, large amounts of glucose are required by lactating dair...
of UDP-galactose synthesis and transport: potential rate-limiting pro-cesses in initiation of milk p...
alpha-Lactalbumin (alpha-LA) is a regulatory protein by which the mammalian beta1,4-galactosyltransf...
$\alpha$-Lactalbumin is the regulatory component of the lactose synthase enzyme complex. It acts to ...
The lacdiNAc sequence GalNAcβ1 → 4GlcNAcβ1-R occurs in the N- and O-glycans of many glycoproteins in...
'To whom correspondence should be addressed The lacdiNAc sequence GalNAcpi->4GlcNAcpi-R occu...
BACKGROUND: The regulation of milk lactose biosynthesis is highly dependent on the action of a speci...
An increasing number of studies have shown that the disaccharide GalNAcβ1→4GlcNAc (LacdiNAc) group b...
The capacity of UDP-N-acetyl-α-D-glucosamine (UDP-GlcNAc) as an in vitro acceptor substrate for β-1,...
beta-1,4-Galactosyltransferase 1 (Gal-T1) transfers galactose (Gal) from UDP-Gal to N-acetylglucosam...
AbstractWe have isolated from bovine colostrum the lactose analog GalNAcβ1→4Glc. The enzymatic basis...
Protein glycosylation is a post-translational modification that produces glycosides linkages through...
Alpha-lactalbumin consists of all the essential and nonessential amino acids, which play a dramatic ...
As the main precursor for lactose synthesis, large amounts of glucose are required by lactating dair...
<p>BMEC were treated for 12 h with various concentration of glucose. Values with different superscri...
As the main precursor for lactose synthesis, large amounts of glucose are required by lactating dair...
of UDP-galactose synthesis and transport: potential rate-limiting pro-cesses in initiation of milk p...
alpha-Lactalbumin (alpha-LA) is a regulatory protein by which the mammalian beta1,4-galactosyltransf...
$\alpha$-Lactalbumin is the regulatory component of the lactose synthase enzyme complex. It acts to ...
The lacdiNAc sequence GalNAcβ1 → 4GlcNAcβ1-R occurs in the N- and O-glycans of many glycoproteins in...
'To whom correspondence should be addressed The lacdiNAc sequence GalNAcpi->4GlcNAcpi-R occu...
BACKGROUND: The regulation of milk lactose biosynthesis is highly dependent on the action of a speci...
An increasing number of studies have shown that the disaccharide GalNAcβ1→4GlcNAc (LacdiNAc) group b...
The capacity of UDP-N-acetyl-α-D-glucosamine (UDP-GlcNAc) as an in vitro acceptor substrate for β-1,...
beta-1,4-Galactosyltransferase 1 (Gal-T1) transfers galactose (Gal) from UDP-Gal to N-acetylglucosam...
AbstractWe have isolated from bovine colostrum the lactose analog GalNAcβ1→4Glc. The enzymatic basis...
Protein glycosylation is a post-translational modification that produces glycosides linkages through...
Alpha-lactalbumin consists of all the essential and nonessential amino acids, which play a dramatic ...
As the main precursor for lactose synthesis, large amounts of glucose are required by lactating dair...
<p>BMEC were treated for 12 h with various concentration of glucose. Values with different superscri...
As the main precursor for lactose synthesis, large amounts of glucose are required by lactating dair...
of UDP-galactose synthesis and transport: potential rate-limiting pro-cesses in initiation of milk p...