Lgr5+ adult intestinal stem cells are highly proliferative throughout life. Single Lgr5+ stem cells can be cultured into three-dimensional organoids containing all intestinal epithelial cell types at near-normal ratios. Conditions to generate the main cell types (enterocyte, goblet cells, Paneth cells, and M cells) are well established, but signals to induce the spectrum of hormone-producing enteroendocrine cells (EECs) have remained elusive. Here, we induce Lgr5+ stem cell quiescence in vitro by blocking epidermal growth factor receptor (EGFR) or mitogen-associated protein kinase (MAPK) signaling pathways in organoids and show that their quiescent state is readily reverted. Quiescent Lgr5+ stem cells acquire a distinct molecular signature ...
The in vitro analysis of intestinal epithelium has been hampered by a lack of suitable culture syste...
AbstractThe cellular composition of barrier epithelia is essential to organismal homoeostasis. In pa...
The work in this thesis adds to our knowledge of intestinal stem cell physiology. After identificati...
Lgr5+ adult intestinal stem cells are highly proliferative throughout life. Single Lgr5+ stem cells ...
The existence and interaction of proliferating and quiescent intestinal stem cells have been debated...
Although Lgr5(+) intestinal stem cells have been expanded in vitro as organoids, homogeneous culture...
AbstractThe small intestine consists of two histological compartments composed of the crypts and the...
Embryonic stem cells, with the ability of self-renewal and differentiation to generate cells of all ...
Homeostasis of self-renewing small intestinal crypts results from neutral competition between Lgr5 s...
The intestinal tract has a rapid epithelial cell turnover, which continues throughout life. The proc...
The mammalian intestine is covered by a single layer of epithelial cells that is renewed every 4-5 d...
Cycling Lgr5+ stem cells fuel the rapid turnover of the adult intestinal epithelium. The existence o...
The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recentl...
The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We originally d...
Critical to the identification of putative stem cell populations is the ability to evalu-ate their f...
The in vitro analysis of intestinal epithelium has been hampered by a lack of suitable culture syste...
AbstractThe cellular composition of barrier epithelia is essential to organismal homoeostasis. In pa...
The work in this thesis adds to our knowledge of intestinal stem cell physiology. After identificati...
Lgr5+ adult intestinal stem cells are highly proliferative throughout life. Single Lgr5+ stem cells ...
The existence and interaction of proliferating and quiescent intestinal stem cells have been debated...
Although Lgr5(+) intestinal stem cells have been expanded in vitro as organoids, homogeneous culture...
AbstractThe small intestine consists of two histological compartments composed of the crypts and the...
Embryonic stem cells, with the ability of self-renewal and differentiation to generate cells of all ...
Homeostasis of self-renewing small intestinal crypts results from neutral competition between Lgr5 s...
The intestinal tract has a rapid epithelial cell turnover, which continues throughout life. The proc...
The mammalian intestine is covered by a single layer of epithelial cells that is renewed every 4-5 d...
Cycling Lgr5+ stem cells fuel the rapid turnover of the adult intestinal epithelium. The existence o...
The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recentl...
The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We originally d...
Critical to the identification of putative stem cell populations is the ability to evalu-ate their f...
The in vitro analysis of intestinal epithelium has been hampered by a lack of suitable culture syste...
AbstractThe cellular composition of barrier epithelia is essential to organismal homoeostasis. In pa...
The work in this thesis adds to our knowledge of intestinal stem cell physiology. After identificati...