Reactive Oxygen Species Driven Angiogenesis by Inorganic Nanorods

The exact mechanism of angiogenesis by europium hydroxide nanorods was unclear. In this study we have showed that formation of reactive oxygen species (H(2)O(2) and O(2)center dot-) is involved in redox signaling pathways during angiogenesis, important for cardiovascular and ischemic diseases. Here we used single-walled carbon nanotube sensor array to measure the single-molecule efflux of H(2)O(2) and a HPLC method for the determination of O(2)center dot- from endothelial cells in response to proangiogenic factors. Additionally, reactive oxygen species-mediated angiogenesis using inorganic nanorods was observed in transgenic (flila:EGFP) zebrafish embryos.This work was supported by National Institutes of Health (NIH) grant HL70567 and CA150190 and a generous gift from Bruce and Martha Atwater to D.M. and the Ramanujan Fellowship grant (SR/S2/RJN-04/2010, GAP 0305\DST\CP) by DST, Govt. of India, New Delhi to C.R.P. R.R. is supported by grants from NIH HL090712 and see funds from Children's Research Institute