Changes in Differentially Expressed Genes in the Liver of Oryzias latipes by Binary Exposure to Carcinogenic Polycyclic Aromatic Hydrocarbons

The biological effects of carcinogenic polycyclic aromatic hydrocarbons (cPAHs) includingbenzo[a]pyrene (BaP), dibenzo[a,h]anthracene (DBA), benzo[a]anthracene (BaA), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF), and indeno[1,2,3-c, d]pyrene (InP) on transcriptomicchanges were determined in the liver of Oryzias latipes. Differentially expressed genes (DEGs)by binary exposure to cPAHs (BaP+BaA, BaP+BbF, BaP+BkF, BaP+DbA, BaP+InP) werescreened by annealing control primers-based polymerase chain reaction followed by sequenceanalysis and BLAST searching. The results showed that four DEGs were commonly expressed bycPAHs and they were identified as ribosomal protein S4, coagulation factor II, elongation factor 1beta, and a predicted protein similar to human immunodeficiency virus type I enhancer bindingprotein 3. This finding suggests that binary exposure to cPAHs interferes protein synthesis requiredfor fundamental liver functions in fish