Development of an anti-aging gel with natural products: hydro-alcoholic extracts and essential oils of two species of Salvia ssp

Salvia officinalis L. and Salvia elegans are perennial plants belonging to Lamiaceae family. New reviews are highlighted pharmacological results attributed to S. officinalis, including anticancer,anti-inflammatory, antioxidant, antimicrobial, antimutagenic, antidementia, hypoglycemic,and hypolipidemic effects. The phytochemicals reported from S. officinalis have been isolated from its essential oil, and infusion preparation. More than 120 components have been characterized in the essential oil of S. officinalis (Ghorbani Esmaeilizadeh, 2017). The objective of this study was to create two anti-ageing gels (Carbopol and Methylcellulose) using essential oil of S. officinalis and hydroalcoholic extracts of S. officinalis and S. elegans. The essential oil was use as preservative and the hydroalcoholic extracts was the bioactive compounds, since it is rich in phenolic compounds with antioxidant capacity. These formulas were put through several tests: stability, physic/chemical, and acute toxicity evaluations. The toxicity test was established using Daphnia magna with objective of LC50 determination after 12 hours. Young daphnids are exposed to a range of concentrations of oil extracts, as well as hydroalcoholic extracts. For S. officinalis five oil concentrations were tested (0.15% to 2.50%) and for S. officinalis and S. ellegans three hydroalcoholic extracts concentrations were tested (1.25%, 2.5%, 5%). Considering S. officinalis, results suggest a decrease toxicity with time exposition, probably due to light or/and oxygen. Considering hydroalcoholic extracts, values of LC50 obtained suggest that, although same pattern, S. ellegans exhibit lower toxicity comparing S. officinalis. Finally, results of Stability Test showed that all samples keep their original properties.