Comparative genomics and transcriptomics of Xanthomonas campestris : Session 3- Physiologie, génétique et génomique des bactéries

The Xanthomonas campestris species causes different diseases on a wide range of Brassicas and is composed of at least four pathovars (campestris, incanae. raphani. unnamed). This species encompasses both vascular and non-vascular pathogens. A comparative genomic analysis of X campestris diversity was performed at the intraspecific and intrapathovar levels by sequencing more than 40 strains. Structural genome annotation was performed and benefited from deep sequencing of small and large RNAs. This approach allowed the exact determination of transcriptional start sites and the identification of small noncoding RNAs. We also used RNA sequencing to characterize the type III regulon in several strains and could identify novel type 111 effectomes. Core and variable genomes and type 111 effectomes were determined. These results evidenced an unsuspected genomic diversity in this species. The latest progress in this project will be presented. (Texte intégral