In vitro binding between glutathione and cloxacillin by a.c. polarography

The interaction of glutathione with cloxacillin was investigated by a new a.c. polarographic procedure. The theoretical treatment used to describe the interaction is strongly consistent with the experimental results. The stability constant, K, and coordination number, p, were calculated from the a.c. polarographic data. Under physiological conditions, we obtained a K value of 3.74×1014 and a p value of 3.12 suggesting 1:3 interaction for the glutathione-cloxacillin species. The coordination number is pH-dependent with a maximum value being obtained under physiological conditions. © 1983 Elsevier Sequoia S.A