Isolation, identification and characterisation of three viruses from Pisum sativum in South Africa

Bibliography: leaves 113-128.Three virus isolates from pea plants (Pisum sativum) from different regions in South Africa were identified and are discussed in this thesis. A number of techniques were used to identify the viruses including serology, electron microscopy, cytopathological studies, aphid transmission tests, host range studies and molecular-based techniques such as PCR, cloning and sequencing and sequence comparisons