An antibacterial hydroxy fusidic acid analogue from Acremonium crotocinigenum

Evans, Liam
Hedger, John N.
Brayford, David
Stavri, Michael
Smith, Eileen
O'Donnell, Gemma
Gray, Alexander I.
Griffith, Gareth W.
Gibbons, Simon

Publication date

October 2006

DOI

10.1016/j.phytochem.2006.06.033

Abstract

A fusidane triterpene, 16-deacetoxy-7-β-hydroxy-fusidic acid (1), was isolated from a fermentation of the mitosporic fungus Acremonium crotocinigenum. Full unambiguous assignment of all 1H and 13C data of 1 was carried out by extensive one- and two-dimensional NMR studies employing HMQC and HMBC spectra. Compound 1 was tested against a panel of multidrug-resistant (MDR) and methicillin-resistant Staphylococcus aureus (MRSA) strains and showed minimum inhibitory concentration values of 16 μg/ml. Bioautography-led isolation of an extract of the fermentation of the mitosporic fungus Acremonium crotocinigenum resulted in the characterisation of the fusidane triterpene related to fusidic acid, 1, as the major anti-staphylococcal natural product....