Laser photoacoustic detection allows in planta detection of nitric oxide in tobacco following challenge with avirulent and virulent pseudomonas syringae pathovars

We demonstrate the use of laser photoacoustic detection (LPAD) as a highly sensitive method to detect in planta nitric oxide (.NO) production from tobacco (Nicotiana tabacum). LPAD calibration against .NO gas demonstrated a linear relationship over 2 orders of magnitude with a detection threshold o