Rapid amplification of cDNA ends (RACE) is a widely used approach for transcript identification. Random clone selection from the RACE mixture, however, is an ineffective sampling strategy if the dynamic range of transcript abundances is large. To improve sampling efficiency of human transcripts, we hybridized the products of the RACE reaction onto tiling arrays and used the detected exons to delineate a series of reverse-transcriptase (RT)-PCRs, through which the original RACE transcript population was segregated into simpler transcript populations. We independently cloned the products and sequenced randomly selected clones. This approach, RACEarray, is superior to direct cloning and sequencing of RACE products because it specifically targe...
As an essential step towards an exhaustive analysis of the coding potential of large regions of the ...
For the analysis of transcriptional tiling arrays we have developed two methods based on state-of-th...
<p>(A) Sketch of the basic principle of the novel single-TCR sequencing strategy. Reverse transcript...
Rapid amplification of cDNA ends (RACE) is a widely used approach for transcript identification. Ran...
Kingdom. RACE (Rapid Amplification of cDNA Ends) is a widely used approach for transcript identifica...
Integrated Genome Browser (IGB) view and rapid amplification of cDNA ends (RACE) products in NB4 RNA...
Rapid Amplification of cDNA Ends (RACE) is a technique used in molecular biology to obtain full leng...
Evidence that the genome is pervasively transcribed into hundreds of thousands different RNAs (1,2) ...
<p>A) For transcript ncINT1Ms2, a single product of 370 bp was obtained with 3′-RACE. After transcri...
High throughput sequencing of the products of 5’ RNA ligase-mediated rapid amplification of cDNA end...
Determination of transcription initiation sites has commonly been performed by primer extension and ...
Low abundance mRNAs are more difficult to examine using microarrays than high abundance mRNAs due to...
In this study we present an improved polymerase chain reaction (PCR)-based methodology to generate l...
Recently, we mapped the sites of transcription across ∼30% of the human genome and elucidated the st...
Long non-coding RNAs (lncRNAs) constitute a large, yet mostly uncharacterized fraction of the mammal...
As an essential step towards an exhaustive analysis of the coding potential of large regions of the ...
For the analysis of transcriptional tiling arrays we have developed two methods based on state-of-th...
<p>(A) Sketch of the basic principle of the novel single-TCR sequencing strategy. Reverse transcript...
Rapid amplification of cDNA ends (RACE) is a widely used approach for transcript identification. Ran...
Kingdom. RACE (Rapid Amplification of cDNA Ends) is a widely used approach for transcript identifica...
Integrated Genome Browser (IGB) view and rapid amplification of cDNA ends (RACE) products in NB4 RNA...
Rapid Amplification of cDNA Ends (RACE) is a technique used in molecular biology to obtain full leng...
Evidence that the genome is pervasively transcribed into hundreds of thousands different RNAs (1,2) ...
<p>A) For transcript ncINT1Ms2, a single product of 370 bp was obtained with 3′-RACE. After transcri...
High throughput sequencing of the products of 5’ RNA ligase-mediated rapid amplification of cDNA end...
Determination of transcription initiation sites has commonly been performed by primer extension and ...
Low abundance mRNAs are more difficult to examine using microarrays than high abundance mRNAs due to...
In this study we present an improved polymerase chain reaction (PCR)-based methodology to generate l...
Recently, we mapped the sites of transcription across ∼30% of the human genome and elucidated the st...
Long non-coding RNAs (lncRNAs) constitute a large, yet mostly uncharacterized fraction of the mammal...
As an essential step towards an exhaustive analysis of the coding potential of large regions of the ...
For the analysis of transcriptional tiling arrays we have developed two methods based on state-of-th...
<p>(A) Sketch of the basic principle of the novel single-TCR sequencing strategy. Reverse transcript...