Pathogenicity traits correlate with the susceptible Vitis vinifera leaf physiology transition in the biotroph fungus Erysiphe necator: An adaptation to plant ontogenic resistance

How and when the pathogen cycle is disrupted during plant development is crucial for harnessing ontogenic resistance in sustainable agriculture. Ontogenic resistance against powdery mildew (Erysiphe necator) was quantified on Vitis vinifera. Shoots were sampled in the vineyard at several dates during seasonal growth and processed in the laboratory under controlled conditions. Experiments were conducted on two susceptible Vitis vinifera Cabernet Sauvignon and Merlot. The process of leaf ontogenic resistance was investigated by measuring three quantitative traits of pathogenicity: the infection efficiency, sporulation and mycelium growth. Morphological and physiological plant indicators were used to identify leaf changes that resulted in ontogenic resistance and to predict pathogen variations that were linked to pathogenicity traits. The process of ontogenic resistance was established early in correspondence with the physiological transition of the leaf from sink to source status and was characterised by its increase in sugar content. The three traits of pathogenicity that we measured were affected, and their variation was strongly correlated with leaf age. Using leaf age, we were able to accurately predict the susceptibility of the leaf: a leaf aged, on average, 13.3 days had a very high probability (0.8) of being susceptible, while this probability decreased to 0.5 one week later. Sporulation was more closely correlated with variations in sugar and the infection efficiency in leaf water. The results for both cultivars were consistent. Ontogenic resistance on grapevine leaves is thus interpreted to be a strong, immutable physiological process that Erysiphe necator is able to circumvent by restricting its development to sink tissue. Future research should explore how this native plant resistance can be incorporated into grape management strategies to better control powdery mildew (PM) epidemics with reduced amounts of fungicides