Simultaneous enrichment and optical detection of low levels of stressed Escherichia coli O157:H7 in food matrices

Mondani, L.
Roupioz, Yoann
Delannoy, S.
Fach, P.
Livache, Thierry

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Publication date

January 2014

DOI

10.1111/jam.12522

Publisher

Wiley

Abstract

International audienceAims: Rapid detection of enterohaemorrhagic E. coli O157:H7 in large range of stress conditions occurring in food processing. Methods and Results: Detection of E. coli O157:H7 in various food processing stress conditions using surface plasmon resonance imaging (SPRi) technique on an antibody microarray was evaluated. The direct detection method based on the culture/capture/measure (CCM) process consists of detecting bacteria during an enrichment step, which significantly decreases the overall assay duration. In optimized culture conditions, this method allows the specific detection of low CFU ml À1 in <7 h. Detection of bacteria directly in contaminated food samples was also conducted. Conclusions: The CCM technique us...

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Simultaneous enrichment and optical detection of low levels of stressed Escherichia coli O157:H7 in food matrices

Abstract

Extracted data

Simultaneous enrichment and optical detection of low levels of stressed Escherichia coli O157:H7 in food matrices

Abstract

Extracted data

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