Microbial transglutaminase (TGase) has been successfully used to produce site-specific protein conjugates derivatized at the level of glutamine (Gln) or lysine (Lys) residues with diverse applications. Here, we study the drug human interferon beta-1a (IFN) as a substrate of TGase. The derivatization reaction was performed using carbobenzoxy-l-glutaminyl-glycine to modify Lys residues and dansylcadaverine for Gln residues. The 166 amino acids polypeptide chain of IFN beta-1a contains 11 Lys and 11 Gln residues potential sites of TGase derivatization. By means of mass spectrometry analyses, we demonstrate the highly selective derivatization of this protein by TGase at the level of Lys115 and as secondary site at the level of Lys33, while no r...
Interferon-alpha (IFNα) is a cytokine that orchestrates innate and adaptive immune responses and pot...
<div><p>The ubiquitin-like protein ISG15 (interferon-stimulated gene of 15 kDa) is strongly induced ...
The transglutaminase (TGase) from Streptomyces mobaraensis catalyzes transamidation reactions in a p...
Microbial transglutaminase (TGase) has been successfully used to produce site-specific protein conju...
Interferon α (IFN α) subtypes are important protein drugs that have been used to treat infectious di...
Interferon α (IFN α) subtypes are important protein drugs that have been used to treat infectious di...
Avidin conjugates have several important applications in biotechnology and medicine. In this work, w...
Avidin conjugates have several important applications in biotechnology and medicine. In this work, w...
Avidin conjugates have several important applications in biotechnology and medicine. In this work, w...
Transglutaminase (TGase, E.C. 2.3.2.13) catalyzes acyl transfer reactions between the gamma-carboxam...
The modification of proteins by chemical methods is well-established, however usually difficult to c...
Transglutaminase (TGase) is able to catalyse the acyl transfer reaction between the \u3b3-carboxamid...
The use of microbial transglutaminase (MTG) to produce site-specific antibody–drug conjugates (ADCs)...
Transglutaminases catalyze transglutamination reactions on glutamines. Transglutaminases are largely...
Poly(ethylene glycol) (PEG) has been widely used to prolong the residence time of proteins in blood ...
Interferon-alpha (IFNα) is a cytokine that orchestrates innate and adaptive immune responses and pot...
<div><p>The ubiquitin-like protein ISG15 (interferon-stimulated gene of 15 kDa) is strongly induced ...
The transglutaminase (TGase) from Streptomyces mobaraensis catalyzes transamidation reactions in a p...
Microbial transglutaminase (TGase) has been successfully used to produce site-specific protein conju...
Interferon α (IFN α) subtypes are important protein drugs that have been used to treat infectious di...
Interferon α (IFN α) subtypes are important protein drugs that have been used to treat infectious di...
Avidin conjugates have several important applications in biotechnology and medicine. In this work, w...
Avidin conjugates have several important applications in biotechnology and medicine. In this work, w...
Avidin conjugates have several important applications in biotechnology and medicine. In this work, w...
Transglutaminase (TGase, E.C. 2.3.2.13) catalyzes acyl transfer reactions between the gamma-carboxam...
The modification of proteins by chemical methods is well-established, however usually difficult to c...
Transglutaminase (TGase) is able to catalyse the acyl transfer reaction between the \u3b3-carboxamid...
The use of microbial transglutaminase (MTG) to produce site-specific antibody–drug conjugates (ADCs)...
Transglutaminases catalyze transglutamination reactions on glutamines. Transglutaminases are largely...
Poly(ethylene glycol) (PEG) has been widely used to prolong the residence time of proteins in blood ...
Interferon-alpha (IFNα) is a cytokine that orchestrates innate and adaptive immune responses and pot...
<div><p>The ubiquitin-like protein ISG15 (interferon-stimulated gene of 15 kDa) is strongly induced ...
The transglutaminase (TGase) from Streptomyces mobaraensis catalyzes transamidation reactions in a p...