Učestalost humanog papilomavirusa 16 i Epstein-Barrova virusa kod bolesnika s oralnim karcinomom pločastih stanica

It has been suggested that certain viruses such as human papillomaviruses (HPV) and Epstein-Barr virus (EBV) might have a role in oral squamous cell carcinoma (OSCC). However, results of the published studies are controversial and are dependent on the geographic distribution and methods of sampling and sample analysis. The aim of this study was to determine the prevalence of HPV 16 and EBV in OSCC patients. In 24 patients with OSCC (mean age 59.6±8.8) and 30 controls (mean age 49.1±8.3), 5 mL of blood was collected to determine the prevalence of EBV by serologic methods. In addition, swabs were obtained to analyze the presence of HPV 16 and EBV by use of polymerase chain reaction. Statistical analysis was performed by use of Mann Whitney test, χ2-test and Spearman correlation test. The results of this study showed that there were no significant differences between OSCC patients and control subjects according to the presence of EBV or HPV 16. Therefore, it can be concludedČini se kako određeni virusi poput humanih papilomavirusa (HPV) i Epstein-Barrova virusa (EBV) mogu imati ulogu u nastanku karcinoma pločastih stanica u usnoj šupljini (OPCK). Ipak, rezultati objavljenih istraživanja na tu temu su kontroverzni i ovise o geografskoj distribuciji i metodama uzimanja uzoraka odnosno raščlambi uzoraka. Cilj ovoga istraživanja je bio odrediti učestalost HPV 16 i EBV u osoba s OPCK. U 24 osobe s OPCK (srednje dobi 59,6±8,8) i u 30 kontrolnih ispitanika (srednje dobi 49,1±8,3) je uzeto 5 mL krvi kako bi se odredio EBV serološkim metodama. Uz to su uzeti i brisevi kako bi se odredila prisutnost HPV 16 i EBV uz pomoć reakcije lančane polimeraze. Statistička analiza je napravljena pomoću Mann-Whitneyeva testa, χ2-testa i Spearmanova korelacijskog testa. Rezultati ovoga istraživanja pokazuju kako nije bilo znakovitih razlika između osoba s OPCK i kontrolnih ispitanika s obzirom na prisutnost HPV 16 ili EBV. Može se zaključiti kako ovi virusi u našoj populaciji oboljelih od OPCK vjerojatno nemaju veliku ulogu