The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host and an insect vector, and adaption of its proteome between lifecycle stages is essential to its survival and virulence. We have optimized a procedure for growing Trypanosoma brucei procyclic form cells in conditions suitable for stable isotope labeling by amino acids in culture (SILAC) and report a comparative proteomic analysis of cultured procyclic form and bloodstream form T. brucei cells. In total we were able to identify 3959 proteins and quantify SILAC ratios for 3553 proteins with a false discovery rate of 0.01. A large number of proteins (10.6%) are differentially regulated by more the 5-fold between lifecycle stages, including those...
Developmental differentiation is a universal biological process that allows cells to adapt to differ...
We describe a single-step centrifugal elutriation method to produce synchronous G1-phase procyclic t...
Developmental differentiation is a universal biological process that allows cells to adapt to differ...
The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host...
The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host...
The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host...
The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host...
The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host...
The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host...
The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host...
Trypanosoma brucei developed a sophisticated life cycle to adapt to different host environments. Alt...
Mass spectrometry based proteomics allows for the identification and quantification of protein and p...
We describe a single-step centrifugal elutriation method to produce synchronous G1-phase procyclic t...
Developmental differentiation is a universal biological process that allows cells to adapt to differ...
We describe a single-step centrifugal elutriation method to produce synchronous G1-phase procyclic t...
Developmental differentiation is a universal biological process that allows cells to adapt to differ...
We describe a single-step centrifugal elutriation method to produce synchronous G1-phase procyclic t...
Developmental differentiation is a universal biological process that allows cells to adapt to differ...
The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host...
The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host...
The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host...
The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host...
The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host...
The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host...
The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a mammalian host...
Trypanosoma brucei developed a sophisticated life cycle to adapt to different host environments. Alt...
Mass spectrometry based proteomics allows for the identification and quantification of protein and p...
We describe a single-step centrifugal elutriation method to produce synchronous G1-phase procyclic t...
Developmental differentiation is a universal biological process that allows cells to adapt to differ...
We describe a single-step centrifugal elutriation method to produce synchronous G1-phase procyclic t...
Developmental differentiation is a universal biological process that allows cells to adapt to differ...
We describe a single-step centrifugal elutriation method to produce synchronous G1-phase procyclic t...
Developmental differentiation is a universal biological process that allows cells to adapt to differ...