Automatic synthesis of phosphopeptides by phosphorylation on the solid phase

An efficient automatic synthesis of phosphopeptides on the solid phase is described. Following completion of their synthesis on the solid support, peptides were phosphorylated using N,N-diisopropyl-bis(4-chlorobenzyl)phosphoramidite. After deprotection, pure phosphopeptides containing a phosphorylated serine or threonine were obtained in high yield