Gaining insight into phosphoproteomes is of the utmost importance for understanding regulation processes such as signal transduction and cellular differentiation. While the identification of phosphotyrosine-containing amino acid sequences in peptides and proteins is now becoming possible, mainly because of the availability of high-affinity antibodies, no general and robust methodology allowing the selective enrichment and analysis of serine- and threonine-phosphorylated proteins and peptides is presently available. The method presented here involves chemical modification of phosphorylated serine or threonine residues and their subsequent derivatization with the aid of a multifunctional probe molecule. The designed probe contains four parts:...
AbstractAlthough numerous strategies have been devised to analyze protein phosphorylation, an abunda...
Peptide synthesis and modification is a versatile chemical biology strategy to construct probes and ...
Proteomics is a rapidly growing area in science and can be defined as the study of the protein profi...
Gaining insight into phosphoproteomes is of the utmost importance for understanding regulation proce...
To improve the detection of phosphorylated peptides/proteins, we developed a novel protocol that inv...
Reversible protein phosphorylation is a key event in numerous biological processes. Mass spectrometr...
Protein phosphorylation is a reversible post-translational modification (PTM)playing a central role ...
Protein phosphorylation is one of the most important and common ways of regulating protein function ...
This chapter focuses on the development of new proteomic approaches based on classical biochemical p...
Reversible protein phosphorylation mediated by kinases, phosphatases, and regulatory molecules is an...
The elucidation of protein post-translational modifications, such as phosphorylation, remains a chal...
Protein phosphorylation is one of the most important and common ways of regulating protein function ...
Protein phosphorylation is one of the most important and common ways of regulating protein function ...
To improve the detection of phosphorylated peptides/proteins, a combination of optimized MS-based st...
Protein phosphorylation is a complex and highly dynamic process involved in numerous biological even...
AbstractAlthough numerous strategies have been devised to analyze protein phosphorylation, an abunda...
Peptide synthesis and modification is a versatile chemical biology strategy to construct probes and ...
Proteomics is a rapidly growing area in science and can be defined as the study of the protein profi...
Gaining insight into phosphoproteomes is of the utmost importance for understanding regulation proce...
To improve the detection of phosphorylated peptides/proteins, we developed a novel protocol that inv...
Reversible protein phosphorylation is a key event in numerous biological processes. Mass spectrometr...
Protein phosphorylation is a reversible post-translational modification (PTM)playing a central role ...
Protein phosphorylation is one of the most important and common ways of regulating protein function ...
This chapter focuses on the development of new proteomic approaches based on classical biochemical p...
Reversible protein phosphorylation mediated by kinases, phosphatases, and regulatory molecules is an...
The elucidation of protein post-translational modifications, such as phosphorylation, remains a chal...
Protein phosphorylation is one of the most important and common ways of regulating protein function ...
Protein phosphorylation is one of the most important and common ways of regulating protein function ...
To improve the detection of phosphorylated peptides/proteins, a combination of optimized MS-based st...
Protein phosphorylation is a complex and highly dynamic process involved in numerous biological even...
AbstractAlthough numerous strategies have been devised to analyze protein phosphorylation, an abunda...
Peptide synthesis and modification is a versatile chemical biology strategy to construct probes and ...
Proteomics is a rapidly growing area in science and can be defined as the study of the protein profi...