Urbanization is a current and increasing threat to biodiversity. The effects of urbanization on the functional and taxonomic composition of macroinvertebrate assemblages were investigated in two seasons along a small urban stream. Species composition was determined by the rate of urbanization; however, response of species richness could not be evidenced. Relative abundance of the sensitive macroinvertebrate groups (EPT%) was negatively related to urbanization. Almost all feeding groups showed a sharp decline in the number of specimens along the increase of urbanization. The study supports the view that urbanization has a negative effect on the biological quality of a stream, yet, this obvious impact can be overridden by different measures s...