Add to ORKGA method for DNA sequencing of ribosomal RNA (rRNA) genes, amplified by polymerase chain reaction (PCR), using internal primers, designed on the basis of conserved regions of rRNA genes for determining a near complete sequence (99%) of the gene using an automated DNA sequencer (Applied Biosystem Incorporation, USA) is described. The procedure is extremely rapid as cloning of the gene is not required for sequence determination. In addition time consuming steps such as ethanol precipitation and hazardous steps such as phenol/chloroform extractions are excluded from the protocol for the purification of extension products after Taq cycle sequencing using the ABI dye terminator chemistry. The method has been successfully used for sequencing of t...
Here we determined the analytical sensitivities of broad-range real-time PCR-based assays employing ...
The invention provides means and methods for efficiently cloning nucleic acid sequences of interest ...
There is a growing appreciation for the role of microbial communities as critical modulators of huma...
A method for DNA sequencing of ribosomal RNA (rRNA) genes, amplified by polymerase chain reaction (P...
The polymerase chain reaction (PCR) is a method for the selective amplification of DNA or RNA segmen...
Using a set of synthetic oligonucleotides homologous to broadly conserved sequences in-vitro amplifi...
New bioactive proteins need to be screened from various microorganisms for the increasing need for i...
One of the major questions in microbial ecology is "who is there?" This question can be answered usi...
Real-time polymerase chain reaction (PCR)-based methodology for the determination of rRNA gene (rrn)...
Oligonucleotide probes targeting the small-subunit rRNA are commonly used to detect and quantify bac...
The polymerase chain reaction (PCR) has made possible the rapid isolation and amplification of speci...
Direct DNA sequencing of amplified polymerase chain reaction (PCR) products offers several advantage...
<p>Primers used in polymerase chain reaction amplification and sequencing of the mitochondrial COI g...
Direct sequencing of polymerase chain reaction (PCR)-generated templates is a commonly used techniqu...
Since the first publication of the dideoxy sequencing method (Ref. [1]), technologies have developed...
Here we determined the analytical sensitivities of broad-range real-time PCR-based assays employing ...
The invention provides means and methods for efficiently cloning nucleic acid sequences of interest ...
There is a growing appreciation for the role of microbial communities as critical modulators of huma...
A method for DNA sequencing of ribosomal RNA (rRNA) genes, amplified by polymerase chain reaction (P...
The polymerase chain reaction (PCR) is a method for the selective amplification of DNA or RNA segmen...
Using a set of synthetic oligonucleotides homologous to broadly conserved sequences in-vitro amplifi...
New bioactive proteins need to be screened from various microorganisms for the increasing need for i...
One of the major questions in microbial ecology is "who is there?" This question can be answered usi...
Real-time polymerase chain reaction (PCR)-based methodology for the determination of rRNA gene (rrn)...
Oligonucleotide probes targeting the small-subunit rRNA are commonly used to detect and quantify bac...
The polymerase chain reaction (PCR) has made possible the rapid isolation and amplification of speci...
Direct DNA sequencing of amplified polymerase chain reaction (PCR) products offers several advantage...
<p>Primers used in polymerase chain reaction amplification and sequencing of the mitochondrial COI g...
Direct sequencing of polymerase chain reaction (PCR)-generated templates is a commonly used techniqu...
Since the first publication of the dideoxy sequencing method (Ref. [1]), technologies have developed...
Here we determined the analytical sensitivities of broad-range real-time PCR-based assays employing ...
The invention provides means and methods for efficiently cloning nucleic acid sequences of interest ...
There is a growing appreciation for the role of microbial communities as critical modulators of huma...
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