Aminobacterium mobile sp. nov., a new anaerobic amino-acid-degrading bacterium

A novel, curved (0.3 x 4.0-5.0 micrometers), Gram-negative, non-sporulating, mesophilic bacterium, designated strain ILE-3t (t = type strain), was isolated from an anaerobic lagoon in a dairy wastewater treatment plant. Optimal growth occured at 37°C and pH 7.4 on a medium containing serine as an energy source and yeast extract. The strain was mobile by means of one or two lateral flagella. It required yeast extract for growth on serine, glycine, threonine and pyruvate. Poor growth was obtained on cysteine, Casamino acids, biotrypcase, peptone and 2-oxoglutarate. In the presence of #Methanobacterium formicicum$, strain ILE-3t oxidized alanine, glutamate, leucine, isoleucine, valine and aspartate to a minor extent. The G+C content of the DNA was 44 mol%. Phylogenetic anlysis of the 16S rRNA gene of strain ILE-3t indicated that it was related to #Aminobacterium colombiense$ (95% similarity value). On the basis of the phenotypic and phylogenetic charactaristics, strain ILE-3t is designated as a new species of the genus #Aminobacterium$, namely #Aminobacterium mobile$ sp. nov. (= DSM 12262t). (Résumé d'auteur