Study of dissociation of human chorionic gonadotropin monoclonal antibody complexes using nitrocellulose as an insoluble support

A method for the determination of real-time kinetic parameters of antigen-antibody reaction using (125)IhCG and immobilized monoclonal antibody (MAb) has already been reported. Improvizations were needed to make it amenable for automation. These included the development of nitrocellulose (NC) as a solid support for the adsorption of MAb, utilization of a lead shield to specifically quantify the radioactivity released in the presence of the radioactive complex and the development of a computer program for analysis of the data. A method was developed for the determination of the kinetic constants with the combined use of the NC adsorbed MAb, (125)IhCG and the lead shield. Kinetic parameters derived from this method compared well with the earlier method, and promise the feasibility of developing an inexpensive indigenous equipment for the measurement of real-time kinetic analysis of ligand-ligate interaction