Identification and characterization of a type II restriction endonuclease, Stri from streptomyces thermodiastaticus

  • Chandrashekaran, Siddamadappa
  • Shankar, Aparna Bhavani
  • Babu, Padmanabhan
  • Paul, Bindu Diana
  • Nagaraja, Valakunja
Publication date
July 1999
Publisher
Indian Academy of Sciences

Abstract

A new type II restriction endonuclease, StrI has been identified from Streptomyces thermodiastaticus. The enzyme has been purified using three column chromatography steps. The enzyme recognizes hexanucleotide sequence and cleaves DNA 5-C down arrow TCGAG-3 as indicated. The optimum temperature, pH, and cation requirements for the enzyme activity were determined

Extracted data

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