Chemical composition of Hyssopus officinalis L. essential oil and its antifungal activity

Chemical composition and antifungal activities of essential oil of Hyssopus officinalis L. and its component β-pinene were done in this paper using macrodilution and microdilution methods. Minimum inhibitory and minimum fungicidal concentrations (MIC and MFC) in ethanol and Tween were determined. Oil and component were investigated against 12 phyto-, animal- and human pathogens; Aspergillus niger, A. ochraceus, A. versicolor, A. flavus, A. terreus, Alternaria alternata, Penicillium ochrochloron, P. funiculosum, Cladosporium cladosporioides, Trichoderma viride, Fusarium tricinctum, Phomopsis helianthi. In essential oil of H. officinalis 27 components were identified (91.70% of total oil). The major compounds were isopinocamphone (43.29%), trans-pinocamphone (16.79%) and β-pinene (16.31%). The essential oil showed strong antifungal activity, with MIC of 2.5 to 3.5 ml/ml in ethanol and Tween. MIC were almost the same in micro- and macrodilution methods, except for Penicillium species where oil dissolved in Tween showed lower MIC. MFC were the same as in macrodilution method. In comparation with bifonazole, essential oil showed much better antifungal potential. .U radu je anliziran hemijski sastav etarskog ulja vrste Hyssopus officinalis i antifungalna aktivnost ukupnog ulja i dominantne komponente β-pinena korišćenjem mikro- i makrodilucione metode. Određivane su minimalne inhibitorne i minimalne fungicidne koncentracije (MIC i MFC) u etanolu i Tween-u. Ispitivana jedinjenja testirana su na 12 vrsta biljnih, životinjskih i humanih patogena: Aspergillus niger, A. ochraceus, A. versicolor, A. flavus, A. terreus, Alternaria alternata, Penicillium ochrochloron, P. funiculosum, Cladosporium cladosporioides, Trichoderma viride, Fusarium tricinctum, Phomopsis helianthi. U etarskom ulju izopa identifikovano je 27 komponenti što predstavlja 91.70% ulja. Dominantne komponente su izopinokamfon (43,29%), trans-pinokamfon (16,79%) i β-pinen (16,31%). Etarsko ulje izopa je pokazalo dobru antifungalnu aktivnost, sa MIC od 2,5 do 3,5 ml/ml u etanolu i Tween-u. U mikrodilucionoj metodi MIC su uglavnom iste kao i u makrodilucionoj, sem za vrste roda Penicillium, kada je ulje rastvoreno u Tween-u (MIC su manje za 0,5 ml/ml). MFC su iste kao MIC u makrodilucionoj metodi. U odnosu na bifonazol, etarsko ulje izopa poseduje veoma jak antifungalni potencijal. .Projekat ministarstva br. 14304