Antimicrobial activity of essential oil of melissa officinalis l. - Lamiaceae

Commercial sample of essential oil of M. officinalis was analysed using GC and GC/MS. The main compounds identified in the essential oil were citronellal (29.3%), limonen (8.84%), geranial (8.70%), a-terpineole (8.02%), a-terpinyl acetate (7.14%) and neral (5.68%). As test organisms the following micromycetes Penicillium ochrochloron, Aspergillus niger Trichoderma viride and bacteria Bacillus subtilis, Staphylococcus epidermidis, Escherichia coli and Micrococcus luteus were used. Microdilution method on microplates 96-system was used for detection of minimum inhibition concentration (MIC), minimum bactericidal (MBC) and fungicidal concentration (MFC). Essential oil did not show antibacterial effect, but the oil of M. officinalis exhibited significant antifungal activity against tested micromycetes.Analiziran je hemijski sastav komercijalnog uzorka etarskog ulja matičnjaka (Melissa officnalis L) i njegova antibakterijska i antifungalna aktivnost. GC i GC-MS analizom konstatovane su najzastupljenije komponente: citronelal (29.3%), limonen (8.84%), geranial (8.70%), a-terpineol (8.02%), a-terpinil acetat (7.14%) i nerol (5.68%). Za ispitivanje biološke aktivnosti korišćeni su test organizmi, mikromicete: Aspergillus niger, Penicillium ochrochloron Trichoderma viride i bakterijski sojevi: Bacillus subtilis, Escherichia coli, Micrococcus luteus i Staphylococcus epidermidis. Mikrodilucionom metodom ispiti-vane su minimalne inhibitorne i mikrobicidne koncentracije. Etarsko ulje matičnjaka je pokazalo značajnu antifungalnu aktivnost ali nizak baktericidni efekat.nul