International audienceIn Southern France as in other parts of Europe, significant changes occurred in settlement patterns between the end of Antiquity and the beginning of the Middle Ages. Small communities gathered to form, by the tenth century, villages organized around a church. This development was the result of a new social and agrarian organization. Its impact on lifestyles and, more precisely, on diet is still poorly understood. The analysis of carbon and nitrogen isotopes in bone collagen from the inhabitants of the well-preserved medieval rural site Missignac-Saint Gilles le Vieux (fifth to thirteenth centuries, Gard, France) provides insight into their dietary practices and enables a discussion about its transformation over time. ...