Short-term effects on soil of biogas digestate, biochar and their combinations

We assessed the suitability of digestate (D) from biogas production and green biochar (B) to improve soil biological activity and antioxidant capacity, and investigated whether there is an interaction between digestate and biochar applied to soil in combination. In a short-term (100-d) laboratory incubation, we monitored soil chemical and biological parameters. We compared soil amendments with 1% D (D1), 5% D (D5), 1% B (B), digestate-biochar combinations (D1+B and D5+B), and soil with no amendment. In D5, CO2 production, antioxidant capacity (TEAC), and dehydrogenase activity (DH-ase) and the contents of microbial biomass C, DOC and alkali-soluble phenols increased to the highest level. The biochar increased the total organic C (TOC) and TEAC of soil but decreased DOC, CO2 production, microbial biomass C and DH-ase. The addition of biochar to digestate reduced soluble compounds (DOC and phenols), thus limiting the amount and activity of the soil microbial biomass (CO2 production and DH-ase). After 100 days of incubation D5+B showed the highest TOC content (82.8% of the initial amount). Both applied alone and in combination with digestate, the biochar appears to enrich the soil carbon sink by reducing CO2 emissions into the atmosphere