Six new bola-DQB alleles sequenced from doayo/namchi (Bos taurus) and akou/white fulani (Bos indicus) cattle

Genomic DNA sarnples were obtained from blood buffy coat preparations ofNarnchi or Doayo (Bos taurus) and Akou or White Fulani (Bos indicus) cattle that were randomly chosen from several herds in the region of Poli, Cameroon for the purpose of identifying liitherto unknown BoLA DQB alleles. Tlie DQB (207 bp) exon 2 fragments were amplified by Polymerase Chain Reaction (PCR). The PCR products were cloned with the Promega pGEMT-T Vector System II kit. Sequencing of the DQB exon 2 clones was perforrned on an Applied Biosytems 377 automated sequencer using 3' ciye labelled dideoxynucleotide triphosphate (Dye terrninator). After verification of the forward and reverse sequences of each allele in six clones of ai least three different animaIs, the arnino acid sequences were compared with aIl the DQB alleles referenced in the official BoLA Nomenclature (http://wvvw2.ri.bbsrc.ac.ukibola/). The new BoLA DQB exon 2 sequences were assigned allele narnes by the BoLA nomenclature committee of the International Society for Animal Genetics. In all six new BoLA-DQB alleles from NamchiiDoayo and Akou "White Fulani cattle were sequenced and given the accession numbers indicated in brackets: CIR1 (AJ421631), CIR2 (AJ421632), CIR3 (AJ421633); CIR4 (AJ421634), CIRS (AJ421635) and CIR6 (AJ421636). CIR1, CIR2 and CIR3 were identified in Doayo and Akou cattle, CIR4 and CIR5 occurred only in Doayo while CIR6 (AJ421636) occurred oruy in Akou cattle. Breedspecifie alleles may be indicators of certain breed-specific characteristics such as disease resistance or susceptibility; so this needs to be investigated further. (Résumé d'auteur