Genetic characterization of citrus rootstocks by using STMS markers : [P24]

STMS markers were used to characterize 41 Citrus rootstocks: A total of 10 Citrus-specific primers were tested, and 9 produced clear, reproducible and discrete bands. Primer Ci03C08 did not give clear amplification and reproducible fragments with all genotypes. The number of fragments amplified from individual Citrus genotypes with each primer pair ranged from one to three. Three fragments per primer pair would be the number expected from triploid Tuzcu M02 Citrange. Primer pair MEST458 from EST library was produced eleven different size fragments (between 203 bp-230 bp). The primer pair MESTl21 and MEST431 from EST library were produced eight (172 bp-202 bp) and six different fragments (322 bp-342 bp) respectively. Primer pair CI02D04B from genomic library was the most informative and polymorphic that produced sixteen fragments with the genotypes studied ranged from 188 bp to 250 bp. Primer pair Ci03G05 and Ci03D12a were produced twelve bands and primer CI02D09 was given thirteen bands. Primer Ci02GI2 and C102A09 from genomic library produced ten and nine bands respectively. Genetic similarity values (Nei and Li, 1979) were calculated and UPGMA (Unweighted pair-group method analysis) cluster analysis was performed to generate a dendogram. The obtained results confirmed the utility of SSR markers to discriminate among Citrus genotypes and to determine the genetic diversity among cultivars. This information is useful for germplasm characterization and identification of cultivars. (Texte intégral